The implementation of electronic signature in EMR of hospital / 中国医学装备

Objective: To gradually implement electronic signature in medical records included outpatient prescription, examination report, test report, hospitalization record and others so as to realize paperless archiving for electronic medical record(EMR) of hospital. Methods: The national law of electronic signature was used as the legal basis and the electronic signature technique was used as the technological foundation to establish electronic signature mechanism about signature, encryption and authentication. The dean who was responsible for informatization of hospital was appointed group leader, and section chief of each relevantly functional department, director of clinical department and head nurse were members of the group. Through perfect the leading group for informatization to ensure electronic signature was effectively implemented. Results: Through adhered to some principles included top-level design, gradual implementation and from easiness to difficulty, the electronic signatures about out-patient prescription, examination reports, test reports, documents of medical record and the paperless archiving for hospitalization record were realized after technique development of many years. Conclusion: The implementation of electronic signature can ensure the legality and authenticity of EMR, and the traceability of modified behavior. And it has realized paperless management for medical archives and has strengthened internal sharing of medical information in hospital.

Mechanical properties of a osteoporotic rat model after treated withXianling Gubao Capsule / 中国组织工程研究

BACKGROUND: The effect of Xianling Gubao Capsule for the elderly female osteoporotic animal models assessed by compression and bending mechanical properties is little reported.OBJECTIVE: To investigate the curative efficacy of Xianling Gubao Capsule, alendronate sodium, calcium carbonate and parathyroid hormone (3-34) for an osteoporotic rat model through compressive and bending tests at the femur.METHODS: The rat models of osteoporosis were established by bilateral ovariectomy, and were then subjected to the treatment of Xianling Gubao Capsule, alendronate sodium, calcium carbonate and parathyroid hormone (3-34),respectively, for 20 weeks. Afterwards, the rats were killed by cutting the abdominal aorta, and then the bilateral femurs were removed to undergo compressive and bending tests.RESULTS AND CONCLUSION: The compressive test: the elastic stress, elastic load, elastic strain, elastic displacement,maximum load, maximum strain, maximum stress, maximum displacement and elastic modulus in the model group were significantly lower than those in the blank control, Xianling Gubao Capsule, alendronate sodium and parathyroid hormone (3-34) groups (P < 0.05). The maximum load, maximum strain, maximum stress, and maximum displacement did not differ significantly between Xianling Gubao Capsule and blank control groups (P > 0.05). All indexes in the compressive and bending tests showed no significant differences between model and calcium carbonate groups (P >0.05). Bending test: the maximum load, maximum stress, maximum strain, maximum bending moment and bending section modulus in the model group were significantly lower than those in the blank control, Xianling Gubao Capsule,alendronate sodium and parathyroid hormone (3-34) groups (P < 0.05). The bending mechanical indexes showed no significant differences between Xianling Gubao Capsule and blank control groups (P > 0.05). To conclude, after compressive and bending tests, Xianling Gubao Capsule, alendronate sodium, and parathyroid hormone (3-34) all restore the mechanical properties of the femur, and Xianling Gubao Capsule obtains a significant curative efficacy.

Cellular sources of interleukin 16 in benign and malignant pleural effusions / 中华医学杂志(英文版)

<p><b>BACKGROUND</b>Interleukin 16 (IL-16) can be detected by ELISA in pleural effusion (PE) and its concentration is higher than in serum. This study investigated the cellular sources of IL-16 in PE.</p><p><b>METHODS</b>The samples of PE were collected from 34 patients who were newly diagnosed having PE in the pleural cavity. We performed cell culture to purify the pleural mesothelial cells (PMC), Wright staining to count the purity and immunocytochemical stain to identify the cultured cells. The intracellular IL-16 expression was detected by flow cytometry (FCM). The different cells in PE were first separated by magnetic cell sorting (MCAS) then the separated cells were cultured in RPMI1640 with 10% fetal calf serum (FCS). We extracted the supernatant and detected IL-16 concentration by ELISA. The IL-16 protein was detected by immunohistochemistry and double immunofluorescence staining.</p><p><b>RESULTS</b>The percentages of cells which secreted IL-16 were: CD3(+)CD8(-) cells ((74.27 ± 15.56)%, n = 34); CD3(+)CD8(+) cells ((69.86 ± 18.55)%, n = 34); CD19(+) cells ((45.30 ± 18.77)%, n = 15); CD14(+) cells ((16.91 ± 16.69)%, n = 15); and PMC ((2.05 ± 1.85)%, n = 7). The concentrations of IL-16 in the supernatant from cultured cells were: CD4(+) cells ((102.50 ± 42.51) ng/L, n = 5); CD8(+) cells ((92.58 ± 18.34) ng/L, n = 5); CD19(+) cells ((79.85 ± 5.62) ng/L, n = 5); CD14(+) cells ((58.51 ± 25.38) ng/L, n = 5); and PMC ((18.14 ± 8.37) ng/L, n = 5). In lymphocytes, monocytes/macrophages and PMC, we could observe the cells that expressed IL-16 protein. In paraffin-embedded sections, we also could observe by immunohistochemistry the CD4(+)IL-16(+) cells, CD8(+)IL-16(+) cells, CD19(+)IL-16(+) cells, and CD14(+)IL-16(+) cells.</p><p><b>CONCLUSIONS</b>IL-16 in PE is mainly secreted by T lymphocytes, including CD3(+)CD8(-) cells and CD3(+)CD8(+) cells. CD19(+) cells and CD14(+) cells can also secrete IL-16, but the percentage of PMC that can secrete IL-16 is very low.</p>

Bio-safety Problem of Cultivating Innovative Talents in Pathogenic Biology / 微生物学通报

We studied on the bio-safety problem of cultivating innovative talents in medical microbiology. The bio-safety of laboratory was controlled by educating bio-safety before the experiments, regulating basic operations during the experiments and constructing management system out of the experiments. Then we got some experience to ensure students’ bio-safety during the research.

Experiment of Exploring Experiment Teaching in Microbiology / 中华医学教育探索杂志

Quality education and innovative ability cultivation of students are a new position in higher education.Exploring exper- iment was applied in teaching of microbiological experiment for enhancing integrative diathesis and cultivating innovative spirit and ability of students.The practice has been proved that learning fervor of students was increased adequately.Unaided operation abili- ty,integrative analysis ability and innovative idea were enhanced,too.Accordingly,teaching quality of microbiological experiment was improved.

Exploring Experiment Teaching and Innovative Ability Cultivating of Students in Medical Microbiology / 微生物学通报

Diathesis education and innovative ability cultivating of students are a new position in higher education.Exploring experiment was applied in medical undergraduates of grade 2004 for enhancing integrative diathesis and cultivating innovative spirit and ability of students.It was proved that application of exploring experiment could increase adequately learning fervor and interest of students.Teaching quality of microbiological experiment was enhanced accordingly.Application of exploring experiment was useful supplement and attempt of traditional teaching mode.